background and objectives: to investigate coagulase gene polymorphisms of mrsa and mssa isolates from shiraz teaching hospitals from 2011 to 2012. materials and methods: a total of 302 isolates of staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. the isolates were subj...

BACKGROUND AND OBJECTIVES To investigate coagulase gene polymorphism of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. MATERIALS AND METHODS A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subje...

Background: Staphylococcus aureus is a common pathogen in human that can be the cause of a wide range of infectious diseases including bacteremia, pneumonia, cellulitis, and osteomyelitis and skin and soft tissue infections. The coagulase enzyme is one of the most important virulence factors of this bacterium. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Coa...

We compared the results of two typing methods for 678 strains of methicillin-resistant Staphylococcus aureus and methicillin-susceptible S. aureus. PCR-restriction fragment length polymorphism typing of the coagulase gene was a more reliable method than coagulase serotyping from the viewpoint of arbekacin resistance.

The present study was aimed to understand the molecular epidemiology of Staphylococcus aureus (54 isolates), isolated from 422 milk samples obtained 108 subclinical mastitis affected cows (CMT positive ≥1+ in at least one quarter). studied using coagulase (coa) gene polymorphism, 16S-23S ribosomal spascer (RS-PCR) polymorphism and Staphylococcal protein A (Spa) typing. produced 7 genotypes 8 RS...

Forty-three methicillin-resistant Staphylococcus aureus (MRSA) isolates with known genetic and epidemiological relatedness and different degrees of transmission were analyzed by antibiotyping, protein A gene polymorphism analysis, and coagulase gene polymorphism analysis. The three typing systems were evaluated for their performance and convenience to define clones and to discriminate between e...

Pulsed-field gel electrophoresis (PFGE) and coagulase gene restriction profile (CRP) analysis techniques were used to analyze 71 Staphylococcus aureus isolates recovered from nine food-borne disease outbreaks. Twenty-two PFGE profiles and 11 CRPs were identified, with discrimination indices of 0.86 and 0.72, respectively. In addition, the variable regions of the coagulase genes of 39 isolates w...

One hundred and ninety eight samples of human and animal sources from different areas in El-Sharkia Governorate were used in this study to isolate Staphylococcus species and to determine the presence of coagulase ( coa) gene,a virulence gene detected in S. aureus (CoPS), by PCR. Primary isolation on mannitol salt agar, $-haemolysis, tube coagulase test (TCT) and other biochemical characterizati...